In Vitro Iodination of Plant Ribonucleic Acids
نویسندگان
چکیده
منابع مشابه
In vitro iodination of plant ribonucleic acids.
The optimum conditions for in vitro iodination of RNAs have been established which yield specific radioactivities ranging from 10 x 10(4) to 10 x 10(6) cpm/mug. A nomogram has been constructed by correlating specific radioactivities of RNA with concentration of KI, RNA, and (125)I. This nomogram can be used to determine the conditions for the desired specific radioactivities for any unknown RNA...
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Iodine monochloride, labeled with 1z51, iodinated ribonucleic acid and deoxyribonucleic acid dissolved as hexadecyltrimethylammonium salts in anhydrous dimethylformamide or N-ethyl acetamide. In the case of RNA (polyuridylate, polycytidylate, and soluble RNA) 5-iodouridine S/-phosphate, 8-iodoguanosine 5’-phosphate, and 5-iodocytidine 5’-phosphate are found. After 24 hours of reaction, 32% of U...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1976
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.58.1.71